Louis van Gaal’s side got off to an exceptional start as Noa Lang twisted, turned, then fired a brilliant strike into the net.
It got better for the Netherlands just a few minutes later when Cody Gakpo doubled their lead guiding a low shot into the bottom corner.
However, Wales got back into the game soon after when Brennan Johnson took his team’s only chance of the first half with a clinical finish.
It seemed to be heading to a 2-1 Netherlands win but suddenly the Welsh were awarded a penalty and Gareth Bale stepped up to convert deep into injury time.
Just as Wales thought they sealed a point, they threw it away against Holland as they did last week when Memphis Depay scored a late winner to spark jubilant scenes.
The Dutch extend their lead to three points in Group D of the Nations League and remain undefeated.